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Human Protein Atlas muc1 protein expression

<t>Mucin</t> <t>1</t> <t>(MUC1)</t> expression in human breast cancer. A , MUC1 expression levels in breast cancer (UALCAN website). B , MUC1 expression levels in different subtypes of breast cancer (UALCAN website). C , MUC1 protein expression in breast cancer (Human Protein Atlas database). D , MUC1 expression in human normal mammary epithelial cells MCF10A, TNBC cells (MDA-MB-231), and TNBC tissues (scale bar=100 μm). Data are reported as means±SE. **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test. BRCA: breast cancer gene; TCGA: The Cancer Genome Atlas; TNBC: triple-negative breast cancer.

Muc1 Protein Expression, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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muc1 protein expression - by Bioz Stars, 2026-07

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1) Product Images from "Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow"

Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

Journal: Brazilian Journal of Medical and Biological Research

doi: 10.1590/1414-431X2026e15075

Mucin 1 (MUC1) expression in human breast cancer. A , MUC1 expression levels in breast cancer (UALCAN website). B , MUC1 expression levels in different subtypes of breast cancer (UALCAN website). C , MUC1 protein expression in breast cancer (Human Protein Atlas database). D , MUC1 expression in human normal mammary epithelial cells MCF10A, TNBC cells (MDA-MB-231), and TNBC tissues (scale bar=100 μm). Data are reported as means±SE. **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test. BRCA: breast cancer gene; TCGA: The Cancer Genome Atlas; TNBC: triple-negative breast cancer.

Figure Legend Snippet: Mucin 1 (MUC1) expression in human breast cancer. A , MUC1 expression levels in breast cancer (UALCAN website). B , MUC1 expression levels in different subtypes of breast cancer (UALCAN website). C , MUC1 protein expression in breast cancer (Human Protein Atlas database). D , MUC1 expression in human normal mammary epithelial cells MCF10A, TNBC cells (MDA-MB-231), and TNBC tissues (scale bar=100 μm). Data are reported as means±SE. **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test. BRCA: breast cancer gene; TCGA: The Cancer Genome Atlas; TNBC: triple-negative breast cancer.

Techniques Used: Expressing, Two Tailed Test

Construction and validation the Mucin 1 (MUC1) knockout cell line 231-MUC1-KO. A , Sequencing results after MUC1 knockout were confirmed by the Snapgene software to identify the monoclonal cell line 231-MUC1-KO. B , RT-qPCR for MUC1 in MDA-MB-231 and 231-MUC1-KO cells. C , Western blot for MUC1 in MDA-MB-231 and 231-MUC1-KO cells. Data are reported as means±SE. ****P<0.0001; two-tailed unpaired Student's t -test.

Figure Legend Snippet: Construction and validation the Mucin 1 (MUC1) knockout cell line 231-MUC1-KO. A , Sequencing results after MUC1 knockout were confirmed by the Snapgene software to identify the monoclonal cell line 231-MUC1-KO. B , RT-qPCR for MUC1 in MDA-MB-231 and 231-MUC1-KO cells. C , Western blot for MUC1 in MDA-MB-231 and 231-MUC1-KO cells. Data are reported as means±SE. ****P<0.0001; two-tailed unpaired Student's t -test.

Techniques Used: Biomarker Discovery, Knock-Out, Sequencing, Software, Quantitative RT-PCR, Western Blot, Two Tailed Test

Effect of Mucin 1 (MUC1) knockout on the proliferation, migration, and invasion of MDA-MB-231 cells. A , MDA-MB-231 cell proliferation by EDU assay (scale bar=100 μm). B , MDA-MB-231 cell proliferation by colony formation assay. C , Migration and invasion of MDA-MB-231 cells by transwell assay (scale bar=150 μm). Data are reported as means±SE. **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test.

Figure Legend Snippet: Effect of Mucin 1 (MUC1) knockout on the proliferation, migration, and invasion of MDA-MB-231 cells. A , MDA-MB-231 cell proliferation by EDU assay (scale bar=100 μm). B , MDA-MB-231 cell proliferation by colony formation assay. C , Migration and invasion of MDA-MB-231 cells by transwell assay (scale bar=150 μm). Data are reported as means±SE. **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test.

Techniques Used: Knock-Out, Migration, EdU Assay, Colony Assay, Transwell Assay, Two Tailed Test

Effect of Mucin 1 (MUC1) knockout on autophagy flow in MDA-MB-231 cells. A , Expression of LC3 I, LC3 II, and P62 proteins by western blot. B , Ad-mCherry-GFP-LC3B-labeled LC3B protein assessed the patency of intracellular autophagy flow (scale bar=100 μm). Data are reported as means±SE. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test. BafA1: Bafilomycin A1; Rapa: Rapamycin.

Figure Legend Snippet: Effect of Mucin 1 (MUC1) knockout on autophagy flow in MDA-MB-231 cells. A , Expression of LC3 I, LC3 II, and P62 proteins by western blot. B , Ad-mCherry-GFP-LC3B-labeled LC3B protein assessed the patency of intracellular autophagy flow (scale bar=100 μm). Data are reported as means±SE. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test. BafA1: Bafilomycin A1; Rapa: Rapamycin.

Techniques Used: Knock-Out, Expressing, Western Blot, Labeling, Two Tailed Test

Effect of Mucin 1 (MUC1) knockout on lysosomes in MDA-MB-231 cells. A , Expression of SNAP29 and STXl7 by western blot. B , Expression of LAMP1 by western blot. C , pH was observed with red fluorescent probe Lysotracker Red (scale bar=100 μm). Data are reported as means±SE. **P<0.01, NS: not significant; two-tailed unpaired Student's t -test.

Figure Legend Snippet: Effect of Mucin 1 (MUC1) knockout on lysosomes in MDA-MB-231 cells. A , Expression of SNAP29 and STXl7 by western blot. B , Expression of LAMP1 by western blot. C , pH was observed with red fluorescent probe Lysotracker Red (scale bar=100 μm). Data are reported as means±SE. **P<0.01, NS: not significant; two-tailed unpaired Student's t -test.

Techniques Used: Knock-Out, Expressing, Western Blot, Two Tailed Test

Schematic diagram of the Mucin 1 (MUC1) oncogenic mechanism in breast cancer cell line MDA-MB-231. Knockout (KO) of MUC1 inhibits the proliferation, migration, and invasion of MDA-MB-231 cells by regulating the autophagic flow, and the possible mechanism may be related to the blocking of the autophagic flow caused by lysosomal damage.

Figure Legend Snippet: Schematic diagram of the Mucin 1 (MUC1) oncogenic mechanism in breast cancer cell line MDA-MB-231. Knockout (KO) of MUC1 inhibits the proliferation, migration, and invasion of MDA-MB-231 cells by regulating the autophagic flow, and the possible mechanism may be related to the blocking of the autophagic flow caused by lysosomal damage.

Techniques Used: Knock-Out, Migration, Blocking Assay

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Journal: Brazilian Journal of Medical and Biological Research

Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

doi: 10.1590/1414-431X2026e15075

Figure Lengend Snippet: Mucin 1 (MUC1) expression in human breast cancer. A , MUC1 expression levels in breast cancer (UALCAN website). B , MUC1 expression levels in different subtypes of breast cancer (UALCAN website). C , MUC1 protein expression in breast cancer (Human Protein Atlas database). D , MUC1 expression in human normal mammary epithelial cells MCF10A, TNBC cells (MDA-MB-231), and TNBC tissues (scale bar=100 μm). Data are reported as means±SE. **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test. BRCA: breast cancer gene; TCGA: The Cancer Genome Atlas; TNBC: triple-negative breast cancer.

Article Snippet: IHC data in the Human Protein Atlas database showed increased MUC1 protein expression in breast cancer tissue, but rarely expressed in normal breast tissue.

Techniques: Expressing, Two Tailed Test

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

doi: 10.1590/1414-431X2026e15075

Figure Lengend Snippet: Construction and validation the Mucin 1 (MUC1) knockout cell line 231-MUC1-KO. A , Sequencing results after MUC1 knockout were confirmed by the Snapgene software to identify the monoclonal cell line 231-MUC1-KO. B , RT-qPCR for MUC1 in MDA-MB-231 and 231-MUC1-KO cells. C , Western blot for MUC1 in MDA-MB-231 and 231-MUC1-KO cells. Data are reported as means±SE. ****P<0.0001; two-tailed unpaired Student's t -test.

Article Snippet: IHC data in the Human Protein Atlas database showed increased MUC1 protein expression in breast cancer tissue, but rarely expressed in normal breast tissue.

Techniques: Biomarker Discovery, Knock-Out, Sequencing, Software, Quantitative RT-PCR, Western Blot, Two Tailed Test

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

doi: 10.1590/1414-431X2026e15075

Figure Lengend Snippet: Effect of Mucin 1 (MUC1) knockout on the proliferation, migration, and invasion of MDA-MB-231 cells. A , MDA-MB-231 cell proliferation by EDU assay (scale bar=100 μm). B , MDA-MB-231 cell proliferation by colony formation assay. C , Migration and invasion of MDA-MB-231 cells by transwell assay (scale bar=150 μm). Data are reported as means±SE. **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test.

Article Snippet: IHC data in the Human Protein Atlas database showed increased MUC1 protein expression in breast cancer tissue, but rarely expressed in normal breast tissue.

Techniques: Knock-Out, Migration, EdU Assay, Colony Assay, Transwell Assay, Two Tailed Test

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

doi: 10.1590/1414-431X2026e15075

Figure Lengend Snippet: Effect of Mucin 1 (MUC1) knockout on autophagy flow in MDA-MB-231 cells. A , Expression of LC3 I, LC3 II, and P62 proteins by western blot. B , Ad-mCherry-GFP-LC3B-labeled LC3B protein assessed the patency of intracellular autophagy flow (scale bar=100 μm). Data are reported as means±SE. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test. BafA1: Bafilomycin A1; Rapa: Rapamycin.

Article Snippet: IHC data in the Human Protein Atlas database showed increased MUC1 protein expression in breast cancer tissue, but rarely expressed in normal breast tissue.

Techniques: Knock-Out, Expressing, Western Blot, Labeling, Two Tailed Test

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

doi: 10.1590/1414-431X2026e15075

Figure Lengend Snippet: Effect of Mucin 1 (MUC1) knockout on lysosomes in MDA-MB-231 cells. A , Expression of SNAP29 and STXl7 by western blot. B , Expression of LAMP1 by western blot. C , pH was observed with red fluorescent probe Lysotracker Red (scale bar=100 μm). Data are reported as means±SE. **P<0.01, NS: not significant; two-tailed unpaired Student's t -test.

Article Snippet: IHC data in the Human Protein Atlas database showed increased MUC1 protein expression in breast cancer tissue, but rarely expressed in normal breast tissue.

Techniques: Knock-Out, Expressing, Western Blot, Two Tailed Test

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

doi: 10.1590/1414-431X2026e15075

Figure Lengend Snippet: Schematic diagram of the Mucin 1 (MUC1) oncogenic mechanism in breast cancer cell line MDA-MB-231. Knockout (KO) of MUC1 inhibits the proliferation, migration, and invasion of MDA-MB-231 cells by regulating the autophagic flow, and the possible mechanism may be related to the blocking of the autophagic flow caused by lysosomal damage.

Article Snippet: IHC data in the Human Protein Atlas database showed increased MUC1 protein expression in breast cancer tissue, but rarely expressed in normal breast tissue.

Techniques: Knock-Out, Migration, Blocking Assay

The expression characteristics of MUC1 in lung adenocarcinoma (LUAD). (A) the expression of MUC1 in normal and LUAD tissues; (B) the expression of MUC1 in different stages; (C) the expression of MUC1 in different T stages; (D) the expression of MUC1 in different N stages; (E) the expression of MUC1 in different M stages; (F) the expression of MUC1 in different genders; (G) the expression of MUC1 in different smoking history; (H) the protein expression of MUC1 in normal and LUAD tissues.

Journal: Annals of Medicine

Article Title: MUC1-EGFR crosstalk with IL-6 by activating NF-κB and MAPK pathways to regulate the stemness and paclitaxel-resistance of lung adenocarcinoma

doi: 10.1080/07853890.2024.2313671

Figure Lengend Snippet: The expression characteristics of MUC1 in lung adenocarcinoma (LUAD). (A) the expression of MUC1 in normal and LUAD tissues; (B) the expression of MUC1 in different stages; (C) the expression of MUC1 in different T stages; (D) the expression of MUC1 in different N stages; (E) the expression of MUC1 in different M stages; (F) the expression of MUC1 in different genders; (G) the expression of MUC1 in different smoking history; (H) the protein expression of MUC1 in normal and LUAD tissues.

Article Snippet: The protein expression of MUC1 in LUAD tissues and normal tissues were retrieved in the Human Protein Atlas (HPA) database ( https://www.proteinatlas.org/ ).

Techniques: Expressing

The immune infiltration analysis of MUC1 expression. (A) The immune cell infiltration in high-MUC1 group and low-MUC1 group; (B) the correlation between MUC1 expression and differential immune cells, the length of the line represents the correlation, the direction of the line represents the positive or negative correlation, and the colour and size of the end point represents p -value.

Journal: Annals of Medicine

Article Title: MUC1-EGFR crosstalk with IL-6 by activating NF-κB and MAPK pathways to regulate the stemness and paclitaxel-resistance of lung adenocarcinoma

doi: 10.1080/07853890.2024.2313671

Figure Lengend Snippet: The immune infiltration analysis of MUC1 expression. (A) The immune cell infiltration in high-MUC1 group and low-MUC1 group; (B) the correlation between MUC1 expression and differential immune cells, the length of the line represents the correlation, the direction of the line represents the positive or negative correlation, and the colour and size of the end point represents p -value.

Article Snippet: The protein expression of MUC1 in LUAD tissues and normal tissues were retrieved in the Human Protein Atlas (HPA) database ( https://www.proteinatlas.org/ ).

Techniques: Expressing

The immune correlation analysis between high-MUC1 group and low-MUC1 group. (A) The effect of MUC1 copy number variation on the immune cells infiltration in LUAD; B-E) the immune score M (B), stromal score (C), tumour purity (D), and ESTIMATE score (E) in high-MUC1 group and low-MUC1 group. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Journal: Annals of Medicine

Article Title: MUC1-EGFR crosstalk with IL-6 by activating NF-κB and MAPK pathways to regulate the stemness and paclitaxel-resistance of lung adenocarcinoma

doi: 10.1080/07853890.2024.2313671

Figure Lengend Snippet: The immune correlation analysis between high-MUC1 group and low-MUC1 group. (A) The effect of MUC1 copy number variation on the immune cells infiltration in LUAD; B-E) the immune score M (B), stromal score (C), tumour purity (D), and ESTIMATE score (E) in high-MUC1 group and low-MUC1 group. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The protein expression of MUC1 in LUAD tissues and normal tissues were retrieved in the Human Protein Atlas (HPA) database ( https://www.proteinatlas.org/ ).

Techniques:

The expression of MUC1, EGFR, and IL-6 in A549 and A549/TAX cells. (A) The expression of MUC1, EGFR, and IL-6 in LUAD cells (A549) and paclitaxel-resistant LUAD cells (A549/TAX); (B) the protein expression of MUC1, EGFR/p-EGFR, and IL-6 in A549 cells and A549/TAX cells; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Journal: Annals of Medicine

Article Title: MUC1-EGFR crosstalk with IL-6 by activating NF-κB and MAPK pathways to regulate the stemness and paclitaxel-resistance of lung adenocarcinoma

doi: 10.1080/07853890.2024.2313671

Figure Lengend Snippet: The expression of MUC1, EGFR, and IL-6 in A549 and A549/TAX cells. (A) The expression of MUC1, EGFR, and IL-6 in LUAD cells (A549) and paclitaxel-resistant LUAD cells (A549/TAX); (B) the protein expression of MUC1, EGFR/p-EGFR, and IL-6 in A549 cells and A549/TAX cells; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The protein expression of MUC1 in LUAD tissues and normal tissues were retrieved in the Human Protein Atlas (HPA) database ( https://www.proteinatlas.org/ ).

Techniques: Expressing

Knock down MUC1 reduces the expression of stem cell pluripotency factor in paclitaxel resistant LUAD cells. (A) screening of effective interference targets for knock down MUC1 in A549 cells and A549/tax cells; (B) cell Counting kit-8 assay (CCK-8) detects the cellular activity of negative control cells (A549/TAX-NC) and MUC1 interfering cells (A549/TAX-siRNA-MUC1); (C) the expression of MUC1, EGFR, and IL-6 in A549/TAX-NC and A549/TAX-siRNA-MUC1 cells; (D) the expression of Oct4, Sox2, and myc in A549/TAX-NC and A549/TAX-siRNA-MUC1 cells. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Journal: Annals of Medicine

Article Title: MUC1-EGFR crosstalk with IL-6 by activating NF-κB and MAPK pathways to regulate the stemness and paclitaxel-resistance of lung adenocarcinoma

doi: 10.1080/07853890.2024.2313671

Figure Lengend Snippet: Knock down MUC1 reduces the expression of stem cell pluripotency factor in paclitaxel resistant LUAD cells. (A) screening of effective interference targets for knock down MUC1 in A549 cells and A549/tax cells; (B) cell Counting kit-8 assay (CCK-8) detects the cellular activity of negative control cells (A549/TAX-NC) and MUC1 interfering cells (A549/TAX-siRNA-MUC1); (C) the expression of MUC1, EGFR, and IL-6 in A549/TAX-NC and A549/TAX-siRNA-MUC1 cells; (D) the expression of Oct4, Sox2, and myc in A549/TAX-NC and A549/TAX-siRNA-MUC1 cells. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The protein expression of MUC1 in LUAD tissues and normal tissues were retrieved in the Human Protein Atlas (HPA) database ( https://www.proteinatlas.org/ ).

Techniques: Knockdown, Expressing, Cell Counting, CCK-8 Assay, Activity Assay, Negative Control

The differentially expressed genes analysis of between A549/Tax-NC and A549/TAX-siRNA-MUC1. (A–B) The volcano plot (A) and heat map (B) of DEGs between A549/Tax-NC and A549/TAX-siRNA-MUC1; (C–E) the expression of ABCB1 (C), ABCC2 (D), and ABCG2 (E) between A549/Tax-NC and A549/TAX-siRNA-MUC1.

Journal: Annals of Medicine

Article Title: MUC1-EGFR crosstalk with IL-6 by activating NF-κB and MAPK pathways to regulate the stemness and paclitaxel-resistance of lung adenocarcinoma

doi: 10.1080/07853890.2024.2313671

Figure Lengend Snippet: The differentially expressed genes analysis of between A549/Tax-NC and A549/TAX-siRNA-MUC1. (A–B) The volcano plot (A) and heat map (B) of DEGs between A549/Tax-NC and A549/TAX-siRNA-MUC1; (C–E) the expression of ABCB1 (C), ABCC2 (D), and ABCG2 (E) between A549/Tax-NC and A549/TAX-siRNA-MUC1.

Article Snippet: The protein expression of MUC1 in LUAD tissues and normal tissues were retrieved in the Human Protein Atlas (HPA) database ( https://www.proteinatlas.org/ ).

Techniques: Expressing

The top 20 DEGs between A549/tax and A549/TAX-siRNA-MUC1.

Journal: Annals of Medicine

Article Title: MUC1-EGFR crosstalk with IL-6 by activating NF-κB and MAPK pathways to regulate the stemness and paclitaxel-resistance of lung adenocarcinoma

doi: 10.1080/07853890.2024.2313671

Figure Lengend Snippet: The top 20 DEGs between A549/tax and A549/TAX-siRNA-MUC1.

Article Snippet: The protein expression of MUC1 in LUAD tissues and normal tissues were retrieved in the Human Protein Atlas (HPA) database ( https://www.proteinatlas.org/ ).

Techniques:

GO and KEGG analysis of DEGs between A549/Tax-NC and A549/TAX-siRNA-MUC1. (A) The GO enrichment of up-regulated DEGs; (B) The KEGG pathway of up-regulated DEGs; (C) The GO enrichment of down-regulated DEGs.

Journal: Annals of Medicine

Article Title: MUC1-EGFR crosstalk with IL-6 by activating NF-κB and MAPK pathways to regulate the stemness and paclitaxel-resistance of lung adenocarcinoma

doi: 10.1080/07853890.2024.2313671

Figure Lengend Snippet: GO and KEGG analysis of DEGs between A549/Tax-NC and A549/TAX-siRNA-MUC1. (A) The GO enrichment of up-regulated DEGs; (B) The KEGG pathway of up-regulated DEGs; (C) The GO enrichment of down-regulated DEGs.

Article Snippet: The protein expression of MUC1 in LUAD tissues and normal tissues were retrieved in the Human Protein Atlas (HPA) database ( https://www.proteinatlas.org/ ).

Techniques:

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